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cd66b fitc  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd66b fitc
    Cd66b Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd66b+fitc/CD66c-PE/pmc13114869-165-17-27
    Average 94 stars, based on 4 article reviews
    cd66b fitc - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: Carbohydrates from Pseudomonas aeruginosa biofilms interact with immune C-type lectins and interfere with their receptor function
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Goat anti-rabbit IgG conjugated to alkaline phosphatase diluted 1:2000 (A3687, Sigma) Recombinant proteins MR-CTLD4-7 (CTLD-4-7-Fc, prepared in house, (ref:35)), DC-SIGN (DC-SIGN-Fc, R&D Systems) and Dectin-2 (Dectin-2-Fc, Enzo) Goat anti-Human IgG conjugated to Alexa fluor 647 (A21445, Invitrogen) Recombinant human MR (CD206) and Dectin-2 with a poly-His-tag were from R&D Systems (Minneapolis, MN).

    Article Title: Flagellin induces myeloid-derived suppressor cells: implications for Pseudomonas aeruginosa infection in cystic fibrosis lung disease.
    Article Snippet: PBMCs were prepared from blood samples by Ficoll density gradient sedimentation (Lymphocyte Separation Medium; Biochrom) and washed twice in RPMI 1640 medium.

    Cytometry:

    Article Title: Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists
    Article Snippet: .. CD14-APC, CD66b-FITC, and CD16-FITC antibodies (Miltenyi Biotec) were used for cytometry analysis of peritoneal cells. ..

    Article Title: Expression of Human CEACAM Receptors Promotes Inflammation and Organ Damage During Systemic Candida albicans Infection in Mice
    Article Snippet: Samples were analyzed with an Axio Observer.Z1/7 microscope using the ZEN 2010 software (both produced by Carl Zeiss Microscopy GmbH, Jena, Germany). .. Human alveolar macrophage analysis: Cells from human bronchoalveolar lavage were stained for CD169-PE (marker for alveolar macrophages), CD66b-FITC (marker for human neutrophils), and CEACAM6 (CD66c-PE-Vio770) (all REA, Miltenyi Biotec, Bergisch Gladbach, Germany) and analyzed on an Attune Acoustic Focusing Cytometer (Life Technologies, Thermo Fisher Scientific, Darmstadt, Germany) using the Attune software v2.1. .. Human peripheral monocyte analysis: Human PBMCs were isolated and stained directly using anti-CD14-FITC (REA, Miltenyi Biotec, Bergisch Gladbach, Germany), anti-CD66c.PE-Vio770 (CEACAM6, REA, Miltenyi Biotec, Bergisch Gladbach, Germany), and eFluor780.

    Isolation:

    Article Title: Granulocytic myeloid derived suppressor cells expand in human pregnancy and modulate T-cell responses.
    Article Snippet: Data acquisition was performed with a FACScalibur flow cytometer (BD Bioscience) and data were analyzed via CellQuest (BD Biosciences). .. For MDSC isolation, cells were obtained from the PBMC fraction and labelled with anti–CD66b-FITC, followed by two sequential anti-FITC magnetic bead separation steps (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. ..

    Article Title: Granulocytic Myeloid-Derived Suppressor Cells Accumulate in Human Placenta and Polarize toward a Th2 Phenotype.
    Article Snippet: Data acquisition was performed with a FACSCalibur flow cytometer (BD Biosciences) and data were analyzed via CellQuest (BD Biosciences). .. For MDSC isolation, cells were obtained from the MNC fraction and labeled with anti–CD66b-FITC, followed by two sequential anti-FITC magnetic bead separation steps (Miltenyi Biotec) according to the manufacturer’s instructions. ..

    Article Title: HLA-G promotes myeloid-derived suppressor cell accumulation and suppressive activity during human pregnancy through engagement of the receptor ILT4.
    Article Snippet: Received: 06 15, 2016; Revised: 07 10, 2016; Accepted: 08 11, 2016 This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1002/eji... This article is protected by copyright.

    Labeling:

    Article Title: Granulocytic Myeloid-Derived Suppressor Cells Accumulate in Human Placenta and Polarize toward a Th2 Phenotype.
    Article Snippet: Data acquisition was performed with a FACSCalibur flow cytometer (BD Biosciences) and data were analyzed via CellQuest (BD Biosciences). .. For MDSC isolation, cells were obtained from the MNC fraction and labeled with anti–CD66b-FITC, followed by two sequential anti-FITC magnetic bead separation steps (Miltenyi Biotec) according to the manufacturer’s instructions. ..

    Staining:

    Article Title: Expression of Human CEACAM Receptors Promotes Inflammation and Organ Damage During Systemic Candida albicans Infection in Mice
    Article Snippet: Samples were analyzed with an Axio Observer.Z1/7 microscope using the ZEN 2010 software (both produced by Carl Zeiss Microscopy GmbH, Jena, Germany). .. Human alveolar macrophage analysis: Cells from human bronchoalveolar lavage were stained for CD169-PE (marker for alveolar macrophages), CD66b-FITC (marker for human neutrophils), and CEACAM6 (CD66c-PE-Vio770) (all REA, Miltenyi Biotec, Bergisch Gladbach, Germany) and analyzed on an Attune Acoustic Focusing Cytometer (Life Technologies, Thermo Fisher Scientific, Darmstadt, Germany) using the Attune software v2.1. .. Human peripheral monocyte analysis: Human PBMCs were isolated and stained directly using anti-CD14-FITC (REA, Miltenyi Biotec, Bergisch Gladbach, Germany), anti-CD66c.PE-Vio770 (CEACAM6, REA, Miltenyi Biotec, Bergisch Gladbach, Germany), and eFluor780.

    Marker:

    Article Title: Expression of Human CEACAM Receptors Promotes Inflammation and Organ Damage During Systemic Candida albicans Infection in Mice
    Article Snippet: Samples were analyzed with an Axio Observer.Z1/7 microscope using the ZEN 2010 software (both produced by Carl Zeiss Microscopy GmbH, Jena, Germany). .. Human alveolar macrophage analysis: Cells from human bronchoalveolar lavage were stained for CD169-PE (marker for alveolar macrophages), CD66b-FITC (marker for human neutrophils), and CEACAM6 (CD66c-PE-Vio770) (all REA, Miltenyi Biotec, Bergisch Gladbach, Germany) and analyzed on an Attune Acoustic Focusing Cytometer (Life Technologies, Thermo Fisher Scientific, Darmstadt, Germany) using the Attune software v2.1. .. Human peripheral monocyte analysis: Human PBMCs were isolated and stained directly using anti-CD14-FITC (REA, Miltenyi Biotec, Bergisch Gladbach, Germany), anti-CD66c.PE-Vio770 (CEACAM6, REA, Miltenyi Biotec, Bergisch Gladbach, Germany), and eFluor780.

    Software:

    Article Title: Expression of Human CEACAM Receptors Promotes Inflammation and Organ Damage During Systemic Candida albicans Infection in Mice
    Article Snippet: Samples were analyzed with an Axio Observer.Z1/7 microscope using the ZEN 2010 software (both produced by Carl Zeiss Microscopy GmbH, Jena, Germany). .. Human alveolar macrophage analysis: Cells from human bronchoalveolar lavage were stained for CD169-PE (marker for alveolar macrophages), CD66b-FITC (marker for human neutrophils), and CEACAM6 (CD66c-PE-Vio770) (all REA, Miltenyi Biotec, Bergisch Gladbach, Germany) and analyzed on an Attune Acoustic Focusing Cytometer (Life Technologies, Thermo Fisher Scientific, Darmstadt, Germany) using the Attune software v2.1. .. Human peripheral monocyte analysis: Human PBMCs were isolated and stained directly using anti-CD14-FITC (REA, Miltenyi Biotec, Bergisch Gladbach, Germany), anti-CD66c.PE-Vio770 (CEACAM6, REA, Miltenyi Biotec, Bergisch Gladbach, Germany), and eFluor780.



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    CD16 + cultured neutrophils were similar to PMNs in mobility, ROS production, phagocytosis, and microbial killing (A) Boxplots and violin plots showing the relative expression of genes and proteins involved in neutrophil effector functions . (B) CD11b/CD18-mediated adhesion assay of PMNs (blue) and SL-II CD16 + neutrophils (pink) to uncoated plastic plates ( n = 4). (C) Chemotactic potential of fluorescently labeled PMNs (blue) and SL-II CD16 + neutrophils (pink) based on movement through filters with a pore size of 3 micron ( n = 5). (D) NADPH oxidase assay to determine the production of extracellular peroxide after the addition of opsonized E. coli , zymosan, STZ, PMA, and PAF/fMLP of PMNs (blue) and SL-II CD16 + neutrophils (pink) ( n = 4 for SL-II CD16 + neutrophils and n = 8 for PMNs). (E) Phagocytosis of either unopsonized or opsonized zymosan by PMNs (blue) versus SL-II CD16 + neutrophils (pink) measured by flow cytometry ( n = 3 for SL-II CD16 + neutrophils and n = 4 for PMNs). (F) Representative images of phagocytosis of either unopsonized or opsonized zymosan labeled with FITC solution (green) by PMNs labeled with calcein red-orange (orange) versus SL-II CD16 + neutrophils labeled with calcein red-orange (orange) at the latest timepoint as visualized by imaging flow cytometric analysis. Scale bar was set at 10 μm ( n = 3). (G and H) Killing of opsonized E. coli and S. aureus, and unopsonized and opsonized C. albicans, respectively, shown for PMNs (blue) ( n = 3) versus SL-II CD16 + neutrophils (pink) ( n = 3). Killing was quantified as the inverse of colony-forming units (CFU) as a percentage relative to the CFU at the start of the assay. Negative values, signifying an increase in the number of colonies were considered to be 0. Data shown in (A–D, H) is represented as median and interquartile range, and (E) and (G) is represented as mean ± SD. p values were calculated using Mann-Whitney U tests and labeled as ∗ p < 0.05 and ∗∗ p < 0.01. n values represent the number of individual donor samples.

    Journal: iScience

    Article Title: Characterization of human CD34 + HSPC-derived neutrophils with limited myeloid-derived immunosuppressive cell activity

    doi: 10.1016/j.isci.2025.113404

    Figure Lengend Snippet: CD16 + cultured neutrophils were similar to PMNs in mobility, ROS production, phagocytosis, and microbial killing (A) Boxplots and violin plots showing the relative expression of genes and proteins involved in neutrophil effector functions . (B) CD11b/CD18-mediated adhesion assay of PMNs (blue) and SL-II CD16 + neutrophils (pink) to uncoated plastic plates ( n = 4). (C) Chemotactic potential of fluorescently labeled PMNs (blue) and SL-II CD16 + neutrophils (pink) based on movement through filters with a pore size of 3 micron ( n = 5). (D) NADPH oxidase assay to determine the production of extracellular peroxide after the addition of opsonized E. coli , zymosan, STZ, PMA, and PAF/fMLP of PMNs (blue) and SL-II CD16 + neutrophils (pink) ( n = 4 for SL-II CD16 + neutrophils and n = 8 for PMNs). (E) Phagocytosis of either unopsonized or opsonized zymosan by PMNs (blue) versus SL-II CD16 + neutrophils (pink) measured by flow cytometry ( n = 3 for SL-II CD16 + neutrophils and n = 4 for PMNs). (F) Representative images of phagocytosis of either unopsonized or opsonized zymosan labeled with FITC solution (green) by PMNs labeled with calcein red-orange (orange) versus SL-II CD16 + neutrophils labeled with calcein red-orange (orange) at the latest timepoint as visualized by imaging flow cytometric analysis. Scale bar was set at 10 μm ( n = 3). (G and H) Killing of opsonized E. coli and S. aureus, and unopsonized and opsonized C. albicans, respectively, shown for PMNs (blue) ( n = 3) versus SL-II CD16 + neutrophils (pink) ( n = 3). Killing was quantified as the inverse of colony-forming units (CFU) as a percentage relative to the CFU at the start of the assay. Negative values, signifying an increase in the number of colonies were considered to be 0. Data shown in (A–D, H) is represented as median and interquartile range, and (E) and (G) is represented as mean ± SD. p values were calculated using Mann-Whitney U tests and labeled as ∗ p < 0.05 and ∗∗ p < 0.01. n values represent the number of individual donor samples.

    Article Snippet: Mouse Anti-Human CD66b Monoclonal antibody, FITC Conjugated (Clone 80H3) , Bio-Rad , Cat #MCA216F; RRID: AB_2077860.

    Techniques: Cell Culture, Expressing, Cell Adhesion Assay, Labeling, Pore Size, Flow Cytometry, Imaging, MANN-WHITNEY